周期素E2重组兔单克隆抗体

产品货号：

BN42073R
中文名称：

周期素E2重组兔单克隆抗体
英文名称：

Rabbit anti-Cyclin E2 Monoclonal antibody
品牌：

Biorigin

货号

产品规格

售价

备注
BN42073R-50ul

50ul

¥2020.00

交叉反应：Human(predicted:Mouse) 推荐应用：WB,IHC-P,IHC-F,ICC,IF,Flow-Cyt
BN42073R-100ul

100ul

¥3240.00

交叉反应：Human(predicted:Mouse) 推荐应用：WB,IHC-P,IHC-F,ICC,IF,Flow-Cyt

产品描述

英文名称	Cyclin E2
中文名称	周期素E2重组兔单克隆抗体
别名	CCN E2; CCNE 2; CCNE2; CCNE2 protein; CYC E2; CYCE 2; CYCE2; CyclinE2; G1/S-specific cyclin E2; G1/S-specific cyclin E2; CCNE2_HUMAN.
研究领域	肿瘤细胞生物染色质和核信号信号转导细胞周期蛋白
抗体来源	Rabbit
克隆类型	Monoclonal
克隆号	2B1
交叉反应	Human, (predicted: Mouse, )
产品应用	WB=1:500-2000 IHC-P=1:50-200 IHC-F=1:50-200 Flow-Cyt=2ug/Test ICC=1:50 IF=1:50-200 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
分子量	44kDa
细胞定位	细胞核
性状	Liquid
浓度	1mg/ml
免疫原	Recombinant human Cyclin E2 protein, around C-terminal 100aa:
亚型	IgG
纯化方法	affinity purified by Protein A
储存液	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed	PubMed
产品介绍	The human Cyclin E2 gene encodes a 404 amino acid protein that is most closely related to Cyclin E. Cyclin E2 mRNA levels peaks at the G1 / S transition. Cyclin E2 associates with Cdk2 in a functional kinase complex that is inhibited by both p27 (Kip1) and p21 (Cip1). Cyclin E2 / Cdk2 phosphorylates histone H1 in vitro. G1 cyclin E controls the initiation of DNA synthesis by activating CDK2. Abnormally high levels of cyclin E expression have frequently been observed in human cancers. Unlike Cyclin E1, which is expressed in great majority of proliferating normal and neoplastically transformed cells, Cyclin E2 levels are low to undetectable in non transformed cells and increase significantly in neoplasm derived cells. Subunit: "Interacts with the CDK2 (in vivo) and CDK3 (in vitro) protein kinases to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex. Subcellular Location: Nucleus. Tissue Specificity: According to PubMed:9858585, highest levels of expression in adult testis, thymus and brain. Lower levels in placenta, spleen and colon. Consistently elevated levels in tumor-derived cells compared to non-transformed proliferating cells. According to PubMed:9840927: low levels in thymus, prostate, brain, skeletal muscle, and kidney. Elevated levels in lung. According to PubMed:9840943 highly expressed in testis, placenta, thymus and brain. In a lesser extent in small intestine and colon. Post-translational modifications: Phosphorylation by CDK2 triggers its release from CDK2 and degradation via the ubiquitin proteasome pathway. Similarity: Belongs to the cyclin family. Cyclin E subfamily. SWISS: O96020 Gene ID: 9134 Database links: Entrez Gene: 9134 Human Entrez Gene: 12448 Mouse Entrez Gene: 362485 Rat Omim: 603775 Human SwissProt: O96020 Human SwissProt: Q7TMS8 Mouse SwissProt: Q9Z238 Mouse Unigene: 567387 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.