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首页>>免疫学>>一抗>>磷脂酰肌醇激酶单克隆抗体
磷脂酰肌醇激酶单克隆抗体
  • 产品货号：
    BN42023M
  • 中文名称：
    磷脂酰肌醇激酶单克隆抗体
  • 英文名称：
    Mouse anti-PI 3 Kinase p85 alpha Monoclonal antibody
  • 品牌：
    Biorigin

  • 货号

    产品规格

    售价

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  • BN42023M-50ul

    50ul

    ¥1486.00

    交叉反应：Human,Mouse,Rat 推荐应用：WB,IHC-P,IHC-F,ICC,IF

  • BN42023M-100ul

    100ul

    ¥2360.00

    交叉反应：Human,Mouse,Rat 推荐应用：WB,IHC-P,IHC-F,ICC,IF

产品描述

英文名称PI 3 Kinase p85 alpha
中文名称磷脂酰肌醇激酶单克隆抗体
别    名PI3-kinase p85 subunit alpha; PI3K p85; GRB1; p50 alpha; p55 alpha; p85 alpha; p85; Phosphatidylinositol 3 kinase associated p85 alpha; Phosphatidylinositol 3 kinase regulatory 1; Phosphatidylinositol 3 kinase regulatory alpha subunit; Phosphoinositide 3 kinase regulatory subunit polypeptide 1 (p85 alpha); PI3 kinase p85 alpha subunit; PI3 kinase p85 subunit alpha; PI3K; PIK3R1; PtdIns 3 kinase p85 alpha; SH3_PI3K_p85alpha; P85A_MOUSE; P85A_HUMAN; PI 3-kinase p85α; PI 3-kinase p85 α; PI 3-kinase p85-α; PI3 kinase P85α.  




研究领域肿瘤  细胞生物  免疫学  信号转导  激酶和磷酸酶  
抗体来源Mouse
克隆类型Monoclonal
克 隆 号5C11
交叉反应Human, Mouse, Rat, 
产品应用WB=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量80kDa
细胞定位细胞核 细胞浆 细胞膜 
性    状Liquid
浓    度1mg/ml
免 疫 原Recombinant mouse PI3K p85 Protein:1-110/724 
亚    型IgG
纯化方法affinity purified by Protein G
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMedPubMed
产品介绍The enzyme phosphatidylinositol 3 kinase (PI3 kinase) is a lipid kinase that generates phosphatidylinositol 3, 4, 5-triphosphate in response to receptor activation in many signal transduction pathways. Class IA PI3Ks exist as a heterodimer of a catalytic 110 kDa (p110) and a regulatory p85 subunit (e.g. p85 alpha). p85 alpha is an adaptor molecule that regulates the activity of the catalytic p110 subunit by binding to phosphorylated receptor tyrosine kinases (RTKs) through its SH2 domain and mediating the interaction between p110 and the plasma membrane. p85 alpha is necessary for insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues.

Function:
Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues.

Subunit:
Heterodimer of a regulatory subunit PIK3R1 and a p110 catalytic subunit (PIK3CA, PIK3CB or PIK3CD). Interacts with FER. Interacts (via SH2 domain) with TEK/TIE2 (tyrosine phosphorylated). Interacts with PTK2/FAK1 (By similarity). Interacts with phosphorylated TOM1L1. Interacts with phosphorylated LIME1 upon TCR and/or BCR activation. Interacts with SOCS7. Interacts with RUFY3. Interacts (via SH2 domain) with CSF1R (tyrosine phosphorylated). Interacts with LYN (via SH3 domain); this enhances enzyme activity. Interacts with phosphorylated LAT, LAX1 and TRAT1 upon TCR activation. Interacts with CBLB. Interacts with HIV-1 Nef to activate the Nef associated p21-activated kinase (PAK). This interaction depends on the C-terminus of both proteins and leads to increased production of HIV. Interacts with HCV NS5A. The SH2 domains interact with the YTHM motif of phosphorylated INSR in vitro. Also interacts with tyrosine-phosphorylated IGF1R in vitro. Interacts with CD28 and CD3Z upon T-cell activation. Interacts with IRS1 and phosphorylated IRS4, as well as with NISCH and HCST. Interacts with FASLG, KIT and BCR. Interacts with AXL, FGFR1, FGFR2, FGFR3 and FGFR4 (phosphorylated). Interacts with FGR and HCK. Interacts with PDGFRA (tyrosine phosphorylated) and PDGFRB (tyrosine phosphorylated). Interacts with ERBB4 (phosphorylated). Interacts with NTRK1 (phosphorylated upon ligand-binding).

Tissue Specificity:
Isoform 2 is expressed in skeletal muscle and brain, and at lower levels in kidney and cardiac muscle. Isoform 2 and isoform 4 are present in skeletal muscle (at protein level).

Post-translational modifications:
Polyubiquitinated in T-cells by CBLB; which does not promote proteasomal degradation but impairs association with CD28 and CD3Z upon T-cell activation.
Phosphorylated. Tyrosine phosphorylated in response to signaling by FGFR1, FGFR2, FGFR3 and FGFR4. Phosphorylated by CSF1R. Phosphorylated by ERBB4. Phosphorylated on tyrosine residues by TEK/TIE2. Dephosphorylated by PTPRJ. Phosphorylated by PIK3CA at Ser-608; phosphorylation is stimulated by insulin and PDGF. The relevance of phosphorylation by PIK3CA is however unclear. Phosphorylated in response to KIT and KITLG/SCF. Phosphorylated by FGR.

Similarity:
Belongs to the PI3K p85 subunit family.
Contains 1 Rho-GAP domain.
Contains 2 SH2 domains.
Contains 1 SH3 domain.

SWISS:
P27986

Gene ID:
18708

Database links:

Entrez Gene: 5295 Human

Entrez Gene: 18708 Mouse

Entrez Gene: 25513 Rat

Omim: 171833 Human

SwissProt: P27986 Human

SwissProt: P26450 Mouse

SwissProt: Q63787 Rat

Unigene: 132225 Human



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications