胰岛素降解酶单克隆抗体

产品货号：

BN41995M
中文名称：

胰岛素降解酶单克隆抗体
英文名称：

Mouse anti-IDE Monoclonal antibody
品牌：

Biorigin

货号

产品规格

售价

备注
BN41995M-50ul

50ul

¥1486.00

交叉反应：Human 推荐应用：WB,IHC-P,IHC-F,ICC,IF
BN41995M-100ul

100ul

¥2360.00

交叉反应：Human 推荐应用：WB,IHC-P,IHC-F,ICC,IF
BN41995M-200ul

200ul

¥3490.00

交叉反应：Human 推荐应用：WB,IHC-P,IHC-F,ICC,IF

产品描述

英文名称	IDE
中文名称	胰岛素降解酶单克隆抗体
别名	BC2; Insulin degrading enzyme; FLJ35968; insulin protease; insulinase; insulysin; Abeta-degrading protease; FLJ35968; Ide; IDE_HUMAN; Insulin-degrading enzyme; OTTHUMP00000020097.
研究领域	肿瘤心血管细胞生物免疫学信号转导生长因子和激素糖尿病
抗体来源	Mouse
克隆类型	Monoclonal
克隆号	5H4
交叉反应	Human,
产品应用	WB=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
分子量	117kDa
细胞定位	细胞浆细胞膜细胞外基质
性状	Liquid
浓度	1mg/ml
免疫原	KLH conjugated synthetic peptide derived from human IDE:
亚型	IgG
纯化方法	affinity purified by Protein G
储存液	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed	PubMed
产品介绍	Insulysin was identified nearly a century ago as an enzyme responsible for the degradation of insulin in cells, although the precise interactions between insulin and insulysin remain elusive. Human insulysin was cloned in 1988, and shown to be a 118 kDa protein that exists primarily as a homodimer, and perhaps also complexed with other molecules. The sequence is well conserved between humans, rats and mice, and the antibody recognizes these species. Insulysin is a metalloproteinase of the clan ME, family M16, which contains an active site HxxEH, a reversal of the canonical HExxH zinc binding motif. Considered a zinc metalloproteinase, the activity of insulysin can be blocked with EDTA or 1-10 phenanthroline. In addition to the active metalloproteinase domain, insulysin contains a second metalloproteinase site which is considered catalytically inactive, and is thought to assist in substrate binding. Insulysin is most closely related to the bacterial proteinase pitrilysin, (the human orthologue of which appears to be MPRP1) and the mammalian proteinsae nardilysin. Generally thought to be a cytoplasmic protein, insulysin has been isolated from many different tissues and cell lines, and can degrade intact insulin, insulin B chain, glucagon, denatured hemoglobin, alpha amyloid protein, TGF alpha and amylin. Recent work implicates insulysin in clearing beta amyloid plaques from the brain, and has generated much interest in Alzheimer’s disease research. The pH optimum for insulysin is basic, pH 8.5, which also distinguishes it from other metalloproteinases. Insulin degrading enzyme (IDE) has a preferential affinity for insulin such that the presence of insulin will inhibit IDE mediated degradation of other substrates. IDE degrades a variety of other peptides including atrial natriuretic peptide and amylin. IDE catabolizes A beta and has been implicated as a candidate enzyme responsible for the degradation and clearance of A beta in the brain. IDE has also been shown to degrade the APP intracellular domain (AICD), a product of gamma secretase cleaved APP that may function in nuclear signaling. Function: Plays a role in the cellular breakdown of insulin, IAPP, glucagon, bradykinin, kallidin and other peptides, and thereby plays a role in intercellular peptide signaling. Degrades amyloid formed by APP and IAPP. May play a role in the degradation and clearance of naturally secreted amyloid beta-protein by neurons and microglia. Subunit: Homodimer. Can form higher oligomers. Interacts (via N-terminus) with varicella-zoster virus (VZV) envelope glycoprotein E (via N-terminus); the membrane-associated isoform may function as an entry receptor for this virus. Subcellular Location: Cytoplasm. Cell surface. Present at the cell surface of neuron cells. The membrane-associated isoform is approximately 5 kDa larger than the known cytosolic isoform. Post-translational modifications: The N-terminus is blocked. Similarity: Belongs to the peptidase M16 family. SWISS: P14735 Gene ID: 3416 Database links: Entrez Gene: 3416 Human Entrez Gene: 15925 Mouse Entrez Gene: 25700 Rat Omim: 146680 Human SwissProt: P14735 Human SwissProt: Q9JHR7 Mouse SwissProt: P35559 Rat Unigene: 500546 Human Unigene: 28366 Mouse Unigene: 45029 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.