RNA结合蛋白LIN28抗体

产品货号：

BN41327R
中文名称：

RNA结合蛋白LIN28抗体
英文名称：

Rabbit anti-Lin28 Polyclonal antibody
品牌：

Biorigin

货号

产品规格

售价

备注
BN41327R-100ul

100ul

¥2360.00

交叉反应：Rat,Mouse(predicted:Sheep,Rabbit,Horse,Cow,Pig,Human) 推荐应用：WB,IHC-P,IHC-F,IF,ELISA
BN41327R-200ul

200ul

¥3490.00

交叉反应：Rat,Mouse(predicted:Sheep,Rabbit,Horse,Cow,Pig,Human) 推荐应用：WB,IHC-P,IHC-F,IF,ELISA

产品描述

英文名称	Lin28
中文名称	RNA结合蛋白LIN28抗体
别名	Lin 28; Lin28A; Lin 28 homolog (C. elegans); Lin 28 homolog A; Lin 28 homolog; Lin-28;y Lin-28 homolog (C. elegans); Lin-28 homolog A; Lin-28A; Lin28; Lin28a; LN28A_HUMAN; Protein lin-28 homolog A; RNA binding protein lin 28; RNA binding protein lin-28; Tex17; ZCCHC1; Zinc finger CCHC domain containing 1; Zinc finger CCHC domain containing protein 1; Zinc finger CCHC domain-containing protein 1; AL024421; CSDD1.
研究领域	细胞生物免疫学发育生物学神经生物学干细胞表观遗传学
抗体来源	Rabbit
克隆类型	Polyclonal
交叉反应	Human, Mouse, Rat, (predicted: Pig, Cow, Horse, Rabbit, Sheep, )
产品应用	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
分子量	29kDa
细胞定位	细胞核细胞浆
性状	Liquid
浓度	1mg/ml
免疫原	KLH conjugated synthetic peptide derived from human Lin28:75-180/209
亚型	IgG
纯化方法	affinity purified by Protein A
储存液	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed	PubMed
产品介绍	LIN-28 is a highly conserved, RNA-binding, cytoplasmic protein. It consists of a cold shock domain and retroviral-type (CCHC) zinc finger motifs that were first identified in Caenorhabditis elegans. LIN-28 controls the timing of events during embryonic development and is readily expressed in embryos, embryonic stem cells and embryonal carcinoma cells. The presence of LIN-28 persists in some adult tissues including cardiac and skeletal muscle. In differentiating myoblasts, LIN-28 increases protein synthesis efficiency and binds to the growth and differentiation factor IGF-II. Function: Acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression (By similarity). Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 in embryonic stem (ES) cells contributes to the maintenance of ES cells. In contrast, LIN28A down-regulation in neural stem cells by miR-125, allows the processing of pre-let-7. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation. Subunit: Monomer. During skeletal muscle differentiation, associated with translation initiation complexes in the polysomal compartment. Directly interacts with EIF3S2. Interaction with NCL is RNA-dependent (By similarity). Interacts with ZCCHC11/TUT4. Subcellular Location: Cytoplasm. Nucleus > nucleolus. Nucleolar localization observed in 10-15% of the nuclei in differentiated myotubes (By similarity). Shuttles between the cytoplasm and the nucleus. Localizes to cytoplasmic processing bodies and stress granules. Tissue Specificity: Expressed in embryonic stem cells (ES cells), placenta and testis. Similarity: Belongs to the lin-28 family. Contains 2 CCHC-type zinc fingers. Contains 1 CSD (cold-shock) domain. SWISS: Q9H9Z2 Gene ID: 79727 Database links: Entrez Gene: 79727 Human Entrez Gene: 83557 Mouse Entrez Gene: 500562 Rat Omim: 611043 Human SwissProt: Q9H9Z2 Human SwissProt: Q8K3Y3 Mouse Unigene: 86154 Human Unigene: 302567 Mouse Unigene: 147538 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.