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首页>>免疫学>>一抗>>细胞周期检查控制蛋白质抗体
细胞周期检查控制蛋白质抗体
  • 产品货号：
    BN41002R
  • 中文名称：
    细胞周期检查控制蛋白质抗体
  • 英文名称：
    Rabbit anti-RAD9 Polyclonal antibody
  • 品牌：
    Biorigin

  • 货号

    产品规格

    售价

    备注

  • BN41002R-100ul

    100ul

    ¥2360.00

    交叉反应：Human,Mouse(predicted:Rat,Chicken,Dog,Pig,Rabbit) 推荐应用：WB,IHC-P,IHC-F,IF,ELISA

  • BN41002R-200ul

    200ul

    ¥3490.00

    交叉反应：Human,Mouse(predicted:Rat,Chicken,Dog,Pig,Rabbit) 推荐应用：WB,IHC-P,IHC-F,IF,ELISA

产品描述

英文名称RAD9
中文名称细胞周期检查控制蛋白质抗体
别    名Cell cycle checkpoint control protein; Cell cycle checkpoint control protein RAD9A; DNA repair exonuclease rad9 homolog A; hRAD 9; hRAD9; Rad 9; RAD 9A; RAD9 (S pombe) homolog; RAD9 homolog A; RAD9 homolog; RAD9A; RAD9A_HUMAN.  
研究领域细胞生物  免疫学  染色质和核信号  信号转导  细胞凋亡  细胞周期蛋白  转录调节因子  
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Mouse,  (predicted: Rat, Chicken, Dog, Pig, Rabbit, )
产品应用WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量43kDa
细胞定位细胞核 
性    状Liquid
浓    度1mg/ml
免 疫 原KLH conjugated synthetic peptide derived from human RAD9 (201-300aa):201-300/391 
亚    型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMedPubMed
产品介绍This gene product is highly similar to Schizosaccharomyces pombe rad9, a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein is found to possess 3' to 5' exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. It forms a checkpoint protein complex with RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade. Use of alternative polyA sites has been noted for this gene. [provided by RefSeq].

Function:
Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase. RAD9A possesses 3'->5' double stranded DNA exonuclease activity. Its phosphorylation by PRKCD may be required for the formation of the 9-1-1 complex.

Subunit:
Component of the toroidal 9-1-1 (RAD9-RAD1-HUS1) complex, composed of RAD9A, RAD1 and HUS1. The 9-1-1 complex associates with LIG1, POLB, FEN1, RAD17, HDAC1, RPA1 and RPA2. The 9-1-1 complex associates with the RAD17-RFC complex. RAD9A interacts with BCL2L1, FEN1, PRKCD, RAD9B, HUS1, RAD1, ABL1, RPA1, ATAD5 and RPA2. Interacts with DNAJC7 and RHNO1.

Subcellular Location:
Nucleus.

Post-translational modifications:
Constitutively phosphorylated on serine and threonine amino acids in absence of DNA damage. Hyperphosphorylated by PRKCD and ABL1 upon DNA damage. Its phosphorylation by PRKCD may be required for the formation of the 9-1-1 complex.

Similarity:
Belongs to the rad9 family.

SWISS:
Q99638

Gene ID:
5883

Database links:

Entrez Gene: 5883 Human

Entrez Gene: 19367 Mouse

Entrez Gene: 100361529 Rat

Omim: 603761 Human

SwissProt: Q99638 Human

SwissProt: Q9Z0F6 Mouse

Unigene: 655354 Human

Unigene: 277629 Mouse



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

Rad9有391个AA构成，Rad9有很多重要生物学功能，如：可调控DNA损伤反应、调节细胞的周期、对损伤的DNA进行修复、诱导细胞凋亡、调控部分基因的转录、具有3’端到5’端核酸外切酵素(exonuclease)的活性、促进核糖核酸的生合成以及参与胚胎发育过程。 曾有报道指出Rad9在调节细胞周期、诱导细胞凋亡及维持整个基因体的完整性等方面的生物功能都和p53极为相似，因此Rad9及p53这两种蛋白在功能上可能具有相互协调作用。