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首页>>免疫学>>一抗>>磷酸化CREB转录共激活因子TORC2抗体
磷酸化CREB转录共激活因子TORC2抗体
  • 产品货号：
    BN40914R
  • 中文名称：
    磷酸化CREB转录共激活因子TORC2抗体
  • 英文名称：
    Rabbit anti-Phospho-SirT1 (Ser27) Polyclonal antibody
  • 品牌：
    Biorigin

  • 货号

    产品规格

    售价

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  • BN40914R-100ul

    100ul

    ¥2470.00

    交叉反应：Human(predicted:Mouse,Rat) 推荐应用：WB,Flow-Cyt,ELISA

产品描述

英文名称Phospho-SirT1 (Ser27)
中文名称磷酸化沉默调节蛋白1抗体
别    名75SirT1; BA57G10.4; hSIR2; hSIRT1; NAD dependent deacetylase SIRT1; NAD dependent deacetylase sirtuin 1; NAD dependent deacetylase sirtuin 2; Silent mating type information regulation 2; OTTHUMP00000198111; OTTHUMP00000198112; SIR2 like 1; SIR2 like protein 1; SIR2-like protein 1; Regulatory protein SIR2 homolog 1; SIR1_HUMAN; SIR2ALPHA; SIR2alpha protein; SIR2L1; SIR2L2; SIRT 1; SIRT-1; Sirt1; SIRT1 Sir2 like proteins (siruitins) type 1; SIRT1: sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae); sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae); SirtT1 75 kDa fragment; sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae);Sirtuin 1; sirtuin; Sirtuin type 1; Sirtuin type 2.  
产品类型磷酸化抗体 
研究领域肿瘤  免疫学  神经生物学  信号转导  细胞凋亡  表观遗传学  
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human,  (predicted: Mouse, Rat, )
产品应用WB=1:500-2000 ELISA=1:5000-10000 Flow-Cyt=1ug/Test 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量58/81kDa
细胞定位细胞核 细胞浆 
性    状Liquid
浓    度1mg/ml
免 疫 原KLH conjugated Synthesised phosphopeptide derived from human SirT1 around the phosphorylation site of Ser27:AS(p-S)PA 
亚    型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMedPubMed
产品介绍This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants.

Function:
SirtT1 75 kDa fragment: catalytically inactive 75SirT1 may be involved in regulation of apoptosis. May be involved in protecting chondrocytes from apoptotic death by associating with cytochrome C and interfering with apoptosome assembly.

Subunit:
Found in a complex with PCAF and MYOD1. Component of the eNoSC complex, composed of SIRT1, SUV39H1 and RRP8. Interacts with HES1, HEY2 and PML. Interacts with RPS19BP1/AROS. Interacts with KIAA1967/DBC1 (via N-terminus); the interaction disrupts the interaction between SIRT1 and p53/TP53. Interacts with SETD7; the interaction induces the dissociation of SIRT1 from p53/TP53 and increases p53/TP53 activity. Interacts with MYCN, NR1I2, CREBZF, TSC2, TLE1, FOS, JUN, NR0B2, PPARG, NCOR, IRS1, IRS2 and NMNAT1. Interacts with HNF1A; the interaction occurs under nutrient restriction. Interacts with SUZ12; the interaction mediates the association with the PRC4 histone methylation complex which is specific as an association with PCR2 and PCR3 complex variants is not found. Interacts with HIV-1 tat.

Subcellular Location:
Nucleus, PML body. Cytoplasm. Note=Recruited to the nuclear bodies via its interaction with PML. Colocalized with APEX1 in the nucleus. May be found in nucleolus, nuclear euchromatin, heterochromatin and inner membrane. Shuttles between nucleus and cytoplasm.
SirtT1 75 kDa fragment: Cytoplasm. Mitochondrion.

Tissue Specificity:
Widely expressed.

Post-translational modifications:
Methylated on multiple lysine residues; methylation is enhanced after DNA damage and is dispensable for deacetylase activity toward p53/TP53.
Phosphorylated. Phosphorylated by STK4/MST1, resulting in inhibition of SIRT1-mediated p53/TP53 deacetylation. Phosphorylation by MAPK8/JNK1 at Ser-27, Ser-47, and Thr-530 leads to increased nuclear localization and enzymatic activity. Phosphorylation at Thr-530 by DYRK1A and DYRK3 acivates deacetylase activity and promotes cell survival. Phosphorylation by mammalian target of rapamycin complex 1 (mTORC1) at Ser-47 inhibits deacetylation activity. Phosphorylated by CaMK2, leading to increased p53/TP53 and NF-kappa-B p65/RELA deacetylation activity (By similarity). Phosphorylation at Ser-27 implicating MAPK9 is linked to protein stability. There is some ambiguity for some phosphosites: Ser-159/Ser-162 and Thr-544/Ser-545.
Proteolytically cleaved by cathepsin B upon TNF-alpha treatment to yield catalytic inactive but stable SirtT1 75 kDa fragment (75SirT1).
S-nitrosylated by GAPDH, leading to inhibit the NAD-dependent protein deacetylase activity (By similarity).

Similarity:
Belongs to the sirtuin family.
Contains 1 deacetylase sirtuin-type domain.

SWISS:
Q96EB6

Gene ID:
23411

Database links:

Entrez Gene: 23411 Human

Entrez Gene: 93759 Mouse

Entrez Gene: 309757 Rat

Omim: 604479 Human

SwissProt: Q96EB6 Human

SwissProt: Q923E4 Mouse

Unigene: 369779 Human

Unigene: 351459 Mouse



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

在Sirtuin蛋白家族中，sirtuin 1参与多种新陈代谢活动，包括DNA的自我保护和修复，抑制脂质过氧化积累，抑制其他细胞凋亡相关基因的表达以及和细胞寿命相关的活动。限制摄入的热量可以加强SIRT1的表达，从而延长了寿命。




























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