货号
产品规格
售价
备注
BN40839R-100ul
100ul
¥1360.00
交叉反应：nan(predicted:Escherichia Coli) 推荐应用：WB,IHC-P,IHC-F,IF,ELISA
产品描述
| 英文名称 | MBP tag |
| 中文名称 | 麦芽糖结合蛋白抗体 |
| 别 名 | MBP-tag; MBP; MMBP; Maltose Binding Protein; Maltose binding protein tag; ECK4026; JW3994; Mal E; MalE; malJ; Maltodextrin binding protein; Maltose ABC transporter periplasmic protein; Maltose binding periplasmic protein; Periplasmic maltose binding protein; MALE_ECOLI. |
| 产品类型 | 标签抗体 |
| 研究领域 | 细胞生物 免疫学 糖蛋白 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Recombinant protein |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 44kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from E. coli Maltose Binding Protein:1-100/396 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 产品介绍 | Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Maltose binding protein(MBP) is the 370 amino acid product of the E.coli mal E gene. MBP is a useful affinity tag that can increase the expression level and solubility of the resulting tagged protein. The MBP tag also promotes proper folding of the attached protein. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be purified in a one step procedure by affinity chromatography cross linked amylose resin. Once bound to amylose, the MBP protein can then be separated from the target protein by cleavage by coagulation Factor Xa at a specific four residue site. Alternatively, the intact fusion protein can be specifically eluted from the resin by the addition of excess free maltose. Subsequent to elution, MBP fusion protein can be visualized either by Western blot analysis or immunoprecipitation using antibodies specific for the MBP-tag. An antibody to MBP can also be used to isolate or detect expression of the protein. Function: Maltose binding protein-MBP is E. coli malE gene encoding the protein, bacterial member of the maltose transport system. malE gene product can integrate with a variety of proteins, it is an effective medium for expression studies. subunit antibody. Subcellular Location: Periplasm. Similarity: Belongs to the bacterial solute-binding protein 1 family. SWISS: P0AEX9 Gene ID: 1039564 Database links: Entrez Gene: 1039564 Escherichia coli Entrez Gene: 4494002 Escherichia coli Entrez Gene: 948538 Escherichia coli Entrez Gene: 960089 Escherichia coli SwissProt: P0AEX9 Escherichia coli SwissProt: P0AEY0 Escherichia coli Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
在线咨询
English
